ARG45149
anti-Dicer antibody
anti-Dicer antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Dicer |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Dicer |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human Dicer. |
| 偶联标记 | Un-conjugated |
| 別名 | DICER1; dicer 1, ribonuclease type III; MNG1; Dicer1e; HERNA; DCR1; EC 3.1.26.3; Helicase with RNase motif; Endoribonuclease Dicer; Helicase MOI; Dicer; K12H4.8-LIKE; RMSE2; KIAA0928; Multinodular Goitre 1; EC 3.1.26 47; RMSE2; GLOW; AviD |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 250 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DICER1 |
| 全名 | dicer 1, ribonuclease type III |
| 背景介绍 | This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. This protein also acts as a strong antiviral agent with activity against RNA viruses, including the Zika and SARS-CoV-2 viruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2021] |
| 生物功能 | Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. [UniProt] |
| 细胞定位 | Cytoplasm; Cytoplasm, perinuclear region. [UniProt] |
| 预测分子量 | 219 kDa |
| 翻译后修饰 | Phosphoprotein. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG45149 anti-Dicer antibody IHC-P image
Immunohistochemistry: Human breast cancer tissue stained with ARG45149 anti-Dicer antibody at 2 μg/ml dilution.
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ARG45149 anti-Dicer antibody WB image
Western blot: 293T stained with ARG45149 anti-Dicer antibody at 0.5 μg/ml dilution.
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ARG45149 anti-Dicer antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45149 anti-Dicer antibody at 1 µg/10^6 cells dilution.
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ARG45149 anti-Dicer antibody WB image
Western blot: C6 stained with ARG45149 anti-Dicer antibody at 0.5 μg/ml dilution.
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ARG45149 anti-Dicer antibody WB image
Western blot: Mouse brain and NIH/3T3 stained with ARG45149 anti-Dicer antibody at 0.5 μg/ml dilution.
