ARG65281
anti-Desmoplakin I antibody
anti-Desmoplakin I antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Desmoplakin isoform I |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | IHC-P |
| 特异性 | This antibody is expected to recognize reported isoform I (NP_004406.2) only. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Desmoplakin I |
| 抗原物种 | Human |
| 抗原 | C-QESVKQAHLRNEH |
| 偶联标记 | Un-conjugated |
| 別名 | 250/210 kDa paraneoplastic pemphigus antigen; Desmoplakin; DP; DPII; DCWHKTA; DPI |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus. [provided by RefSeq, Jul 2008] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 332 kDa |
| 翻译后修饰 | Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments. |
检测图片 (1) Click the Picture to Zoom In
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ARG65281 anti-Desmoplakin antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Heart. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65281 anti-Desmoplakin (aa 1672 -1684) antibody at 3.8 µg/ml dilution followed by AP-staining.
