ARG57056
anti-DYNLL1 / PIN antibody [13C9]
anti-DYNLL1 / PIN antibody [13C9] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [13C9] recognizes DYNLL1 / PIN |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 13C9 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | DYNLL1 / PIN |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment corresponding to aa. 1-89 of Human DYNLL1 / PIN. |
| 偶联标记 | Un-conjugated |
| 別名 | LC8a; Dynein light chain 1, cytoplasmic; PIN; DNCLC1; Protein inhibitor of neuronal nitric oxide synthase; LC8; DLC8; 8 kDa dynein light chain; DLC1; DNCL1; Dynein light chain LC8-type 1; hdlc1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DYNLL1 |
| 全名 | dynein, light chain, LC8-type 1 |
| 背景介绍 | Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Binds and inhibits the catalytic activity of neuronal nitric oxide synthase. Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1. Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity. [UniProt] |
| 预测分子量 | 10 kDa |
| 翻译后修饰 | Phosphorylation at Ser-88 appears to control the dimer-monomer transition. According to PubMed:15193260, it is phosphorylated at Ser-88 by PAK1, however, according to PubMed:18650427, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro. |
检测图片 (1) Click the Picture to Zoom In
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ARG57056 anti-DYNLL1 / PIN antibody [13C9] WB image
Western blot: 40 µg of 1) HeLa, 2) A549, 3) 293T and 4) HepG2 cell lysates stained with ARG57056 anti-DYNLL1 / PIN antibody [13C9] at 1:1000 dilution.
