ARG55249
anti-DUX4 antibody [P4H2]
anti-DUX4 antibody [P4H2] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody
概述
| 产品描述 | Mouse Monoclonal antibody [P4H2] recognizes DUX4 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 特异性 | This antibody recognizes an epitope at the C-terminus of human DUX4. It does not cross-react with DUX4c. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | P4H2 |
| 同位型 | IgG1 |
| 靶点名称 | DUX4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around 76 aa (C-terminus) of Human DUX4 with GST tag. |
| 偶联标记 | Un-conjugated |
| 別名 | Double homeobox protein 10; Double homeobox protein 4; DUX4L |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human testis lysate. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4). |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DUX4 |
| 全名 | double homeobox 4 |
| 背景介绍 | This gene is located within a D4Z4 repeat array in the subtelomeric region of chromosome 4q. The D4Z4 repeat is polymorphic in length; a similar D4Z4 repeat array has been identified on chromosome 10. Each D4Z4 repeat unit has an open reading frame (named DUX4) that encodes two homeoboxes; the repeat-array and ORF is conserved in other mammals. The encoded protein has been reported to function as a transcriptional activator of paired-like homeodomain transcription factor 1 (PITX1; GeneID 5307). Contraction of the macrosatellite repeat causes autosomal dominant facioscapulohumeral muscular dystrophy (FSHD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
| 生物功能 | Involved in transcriptional regulation. May regulate microRNA (miRNA) expression. [UniProt] |
| 研究领域 | Developmental Biology antibody |
| 预测分子量 | 45 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG55249 anti-DUX4 antibody [P4H2] WB image
Western blot: C2C12 cells transfected with pCS2-DUX4 stained with ARG55249 anti-DUX4 antibody [P4H2] at 1:10000 dilution. There is a doublet because the expression construct contains an upstream alternate translation start codon. The lower band is the canonical size for DUX4.
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ARG55249 anti-DUX4 antibody [P4H2] ICC/IF image
Immunofluorescence: Rhabdomyosarcoma cell line RD transfected with pCS2-DUX4, stained with ARG55249 anti-DUX4 antibody [P4H2] (green) at 1:10000 dilution. Cells were counterstained with DAPI (blue) for nuclei.
