ARG57052
anti-DUSP3 antibody [9E6]
anti-DUSP3 antibody [9E6] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [9E6] recognizes DUSP3 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 9E6 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | DUSP3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-185 of Human DUSP3. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.1.3.16; Vaccinia H1-related phosphatase; Dual specificity protein phosphatase 3; EC 3.1.3.48; VHR; Dual specificity protein phosphatase VHR |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P51452 Human Dual specificity protein phosphatase 3 |
|---|---|
| 基因名称 | DUSP3 |
| 全名 | dual specificity phosphatase 3 |
| 背景介绍 | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008] |
| 生物功能 | Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. [UniProt] |
| 预测分子量 | 20 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG57052 anti-DUSP3 antibody [9E6] WB image
Western blot: 40 µg of 1) HeLa cell lysate, 2) HepG2 cell lysate, 3) Jurkat cell lysate, 4) MCF7 cell lysate, 5) 293T cell lysate, 6) U87MG cell lysate, 7) K562 cell lysate stained with ARG57052 anti-DUSP3 antibody [9E6] at 1:3000.
