ARG40852
anti-DPYSL2 / CRMP2 phospho (Thr555) antibody [M539]
anti-DPYSL2 / CRMP2 phospho (Thr555) antibody [M539] for Western blot and Rat
概述
| 产品描述 | Mouse Monoclonal antibody [M539] recognizes DPYSL2 / CRMP2 phospho (Thr555) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | M539 |
| 同位型 | IgG1 |
| 靶点名称 | DPYSL2 / CRMP2 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide (coupled to carrier protein) around Thr555 of Human CRMP2. |
| 偶联标记 | Un-conjugated |
| 別名 | Unc-33-like phosphoprotein 2; Dihydropyrimidinase-related protein 2; Collapsin response mediator protein 2; DRP2; ULIP2; N2A3; DRP-2; DHPRP2; ULIP-2; CRMP-2; CRMP2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P47942 Rat Dihydropyrimidinase-related protein 2 |
|---|---|
| 基因名称 | DPYSL2 |
| 全名 | dihydropyrimidinase-like 2 |
| 背景介绍 | This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011] |
| 生物功能 | Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Membrane. Note=Tightly but non-covalently associated with membranes. [UniProt] |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | 3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509. Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding leading to destabilization of microtubule assembly in axons and neurodegeneration (By similarity). Phosphorylation by DYRK2 at Ser-522 is required for subsequent phosphorylation by GSK3B. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40852 anti-DPYSL2 / CRMP2 phospho (Thr555) antibody [M539] WB image
Western blot: PC-12 cells stimulated with calyculin A (100 nM) for 30 min. The blots were untreated (left) or treated with lambda phosphatase (right) and stained with ARG40852 anti-DPYSL2 / CRMP2 phospho (Thr555) antibody [M539].
