ARG45212
anti-DPH5 antibody
anti-DPH5 antibody for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DPH5 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DPH5 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human DPH5. |
| 偶联标记 | Un-conjugated |
| 別名 | DPH5; Diphthamide Biosynthesis 5; Diphthine Methyl Ester Synthase; CGI-30; Diphthamide Biosynthesis Methyltransferase; DPH5 Homolog (S. Cerevisiae); Diphthine Synthase; Protein X 0011; DPH5 Homolog; EC 2.1.1.314; EC 2.1.1.98; HSPC143; AD-018; NEDSFF; NPD015 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 37 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DPH5 |
| 全名 | Diphthamide Biosynthesis 5 |
| 背景介绍 | Diphthine synthase is an enzyme that in humans is encoded by the DPH5 gene. This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. |
| 生物功能 | S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.. [UniProt] |
| 细胞定位 | cytosol. [UniProt] |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | Phosphoprotein. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG45212 anti-DPH5 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45212 anti-DPH5 antibody at 5 ug/ml dilution.
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ARG45212 anti-DPH5 antibody WB image
Western blot: 293T, HepG2, K562, and Daudi stained with ARG45212 anti-DPH5 antibody at 0.5 μg/ml dilution.
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ARG45212 anti-DPH5 antibody FACS image
Flow Cytometry: A549 stained with ARG45212 anti-DPH5 antibody at 1 µg/10^6 cells dilution.
