ARG63676

anti-DOK5 antibody

anti-DOK5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes DOK5
反应物种 Hu
预测物种 Ms, Rat, Cow, Dog, Pig
应用 IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 DOK5
抗原物种 Human
抗原 CLHRTETFPAYRSEH
偶联标记 Un-conjugated
別名 IRS6; IRS-6; Docking protein 5; C20orf180; Insulin receptor substrate 6; Downstream of tyrosine kinase 5

应用说明

应用建议
应用 推荐稀释比
IHC-P1 - 2 µg/ml
WB1 - 3 µg/ml
应用说明 IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 55816 Human DOK5

Swiss-port # Q9P104 Human Docking protein 5

背景介绍 The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP. [provided by RefSeq, Jul 2008]
研究领域 Signaling Transduction antibody
预测分子量 35 kDa
翻译后修饰 Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.

检测图片 (2) Click the Picture to Zoom In

  • ARG63676 anti-DOK5 antibody WB image

    Western Blot: 1). Mock transfection; 2) DOK5 (RC211718) expressing plasmid transfected HEK293 cell lysate standed with ARG63676 anti-DOK5 antibody

  • ARG63676 anti-DOK5 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Tonsil. (Microwaved antigen retrieval with citrate buffer pH6) stained with ARG63676 anti-DOK5 antibody at 1 µg/ml dilution followed by HRP-staining.