ARG51759
anti-DOK2 phospho (Tyr299) antibody
anti-DOK2 phospho (Tyr299) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DOK2 phospho (Tyr299) |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DOK2 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y(p)-A-V) derived from Human p56Dok-2. |
| 偶联标记 | Un-conjugated |
| 別名 | p56dok-2; Docking protein 2; p56DOK; dok-2; Downstream of tyrosine kinase 2; p56 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DOK2 |
| 全名 | docking protein 2, 56kDa |
| 背景介绍 | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation |
| 生物功能 | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity). [UniProt] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity). |
检测图片 (4) Click the Picture to Zoom In
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ARG51759 anti-DOK2 phospho (Tyr299) antibody WB image
Western blot: Extracts from Jurkat cells untreated or treated with insulin stained with ARG51759 anti-DOK2 phospho (Tyr299) antibody.
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ARG51759 anti-DOK2 phospho (Tyr299) antibody WB image
Western blot: Extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), stained with ARG51759 anti-DOK2 phospho (Tyr299) antibody.
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ARG51759 anti-DOK2 phospho (Tyr299) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51759 anti-DOK2 phospho (Tyr299) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51759 anti-DOK2 phospho (Tyr299) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51759 anti-DOK2 phospho (Tyr299) antibody.
