ARG58004
anti-DOK1 / p62 Dok antibody
anti-DOK1 / p62 Dok antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DOK1 / p62 Dok |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DOK1 / p62 Dok |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human DOK1. |
| 偶联标记 | Un-conjugated |
| 別名 | dok; p62; P62DOK; Docking protein 1; pp62; Downstream of tyrosine kinase 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | WB: Jurkat cell lysate. | ||||||
| 实际分子量 | ~ 62 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DOK1 |
| 全名 | docking protein 1, 62kDa (downstream of tyrosine kinase 1) |
| 背景介绍 | The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010] |
| 生物功能 | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. [UniProt] |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58004 anti-DOK1 / p62 Dok antibody ICC/IF image
Immunofluorescence: K562 cells stained with ARG58004 anti-DOK1 / p62 Dok antibody at 10 µg/ml dilution.
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ARG58004 anti-DOK1 / p62 Dok antibody WB image
Western blot: Jurkat and THP-1 cell lysates stained with ARG58004 anti-DOK1 / p62 Dok antibody at 1 µg/ml dilution.
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ARG58004 anti-DOK1 / p62 Dok antibody ICC/IF image
Immunocytochemistry: K562 cells stained with ARG58004 anti-DOK1 / p62 Dok antibody at 2 µg/ml dilution.
