ARG46129
anti-DOCK8 antibody
anti-DOCK8 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DOCK8 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DOCK8 |
| 抗原物种 | Human |
| 抗原 | A 17 amino acid synthetic peptide within the last 50 amino acids of human DOCK8. |
| 偶联标记 | Un-conjugated |
| 別名 | DOCK8; ZIR8; Dedicator of cytokinesis protein 8; HEL-205; MRD2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 220,230 kDa |
属性
| 纯化 | Affinity chromatography purified |
|---|---|
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8C147 Mouse Dedicator of cytokinesis protein 8 Swiss-port # Q8NF50 Human Dedicator of cytokinesis protein 8 |
|---|---|
| 基因名称 | DOCK8 |
| 全名 | dedicator of cytokinesis 8 |
| 背景介绍 | This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010] |
| 生物功能 | Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed:28028151, PubMed:22461490). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed:28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed:25762780). [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note=Enriched and co-localizes with GTPase CDC42 at the immunological synapse formed during T cell/antigen presenting cell cognate interaction. Translocates from the cytoplasm to the plasma membrane in response to chemokine CXCL12/SDF-1-alpha stimulation. [UniProt] |
| 预测分子量 | 166,220,231 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG46129 anti-DOCK8 antibody IHC-P image
Immunohistochemistry: Human spleen stained with ARG46129 anti-DOCK8 antibody.
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ARG46129 anti-DOCK8 antibody WB image
Western blot: EL4 stained with ARG46129 anti-DOCK8 antibody.
