ARG59503
anti-DNA polymerase beta antibody
anti-DNA polymerase beta antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DNA polymerase beta |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DNA polymerase beta |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-335 of Human DNA polymerase beta (NP_002681.1). |
| 偶联标记 | Un-conjugated |
| 別名 | EC 4.2.99.-; EC 2.7.7.7; DNA polymerase beta |
应用说明
| 预测反应性说明 | Rat | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Jurkat | ||||||||
| 实际分子量 | 37 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | POLB |
| 全名 | polymerase (DNA directed), beta |
| 背景介绍 | The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011] |
| 生物功能 | Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Note=Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage. [UniProt] |
| 预测分子量 | 38 kDa |
| 翻译后修饰 | Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG59503 anti-DNA polymerase beta antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG59503 anti-DNA polymerase beta antibody.
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ARG59503 anti-DNA polymerase beta antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG59503 anti-DNA polymerase beta antibody at 1:1000 dilution.
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ARG59503 anti-DNA polymerase beta antibody WB image
Western blot: 25 µg of extracts from normal (control) and POLB knockout (KO) HeLa cells, using ARG59503 anti-DNA polymerase beta antibody at 1:1000 dilution.
