ARG62468
anti-DNA polymerase beta antibody [18S]
anti-DNA polymerase beta antibody [18S] for Immunohistochemistry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Hamster,Xenopus laevis
Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody [18S] recognizes DNA polymerase beta |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov, Hm, Xenopus laevis |
| 应用 | IHC, IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 18S |
| 同位型 | IgG1 |
| 靶点名称 | DNA polymerase beta |
| 抗原物种 | Rat |
| 抗原 | Rat DNA polymerase beta full length protein (Rat) |
| 偶联标记 | Un-conjugated |
| 別名 | EC 4.2.99.-; EC 2.7.7.7; DNA polymerase beta |
应用说明
| 应用说明 | WB: 1-2 ug/ml IHC: 1/10-1/500 IP: 2 ug/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 1X PBS buffer with sodium azide (< 0.1%). |
| 抗菌剂 | sodium azide (< 0.1%) |
| 浓度 | 2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Polb |
| 全名 | polymerase (DNA directed), beta |
| 背景介绍 | The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011] |
| 生物功能 | Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 38 kDa |
| 翻译后修饰 | Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation. |
克隆号文献
Base excision repair defects invoke hypersensitivity to PARP inhibition.
IP / Mouse
Differential regulation of S-region hypermutation and class-switch recombination by noncanonical functions of uracil DNA glycosylase.
An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells.
WB / Human
Oxidative stress alters base excision repair pathway and increases apoptotic response in apurinic/apyrimidinic endonuclease 1/redox factor-1 haploinsufficient mice.
WB / Mouse
DNA polymerase beta is able to repair breaks in switch regions and plays an inhibitory role during immunoglobulin class switch recombination.
WB, ChIP / Mouse
A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment.
ChIP / Human
Oxidative DNA damage and repair in experimental atherosclerosis are reversed by dietary lipid lowering.
WB / Rabbit
