ARG45477
anti-DNA polymerase alpha / POLA antibody
anti-DNA polymerase alpha / POLA antibody for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DNA polymerase alpha / POLA |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DNApolymerasealpha / POLA |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human DNA polymerase alpha / POLA. |
| 偶联标记 | Un-conjugated |
| 別名 | POLA1; DNA Polymerase Alpha 1, Catalytic Subunit; P180; POLA; Polymerase (DNA) Alpha 1, Catalytic Subunit; DNA Polymerase Alpha Catalytic Subunit P180; DNA Polymerase Alpha Catalytic Subunit; EC 2.7.7.7 47; NSX; N Syndrome (Mental Retardation, Malformations, Chromosome Breakage); Polymerase (DNA Directed), Alpha 1, Catalytic Subunit; Polymerase (DNA-Directed), Alpha (70kD); Polymerase (DNA Directed), Alpha; VEODS; PDR |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 180 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P09884 Human DNA polymerase alpha catalytic subunit |
|---|---|
| 基因名称 | POLA1 |
| 全名 | DNA Polymerase Alpha 1, Catalytic Subunit |
| 背景介绍 | This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010 |
| 生物功能 | Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses [UniProt] |
| 细胞定位 | Cytoplasm; Nucleus. [UniProt] |
| 预测分子量 | 166 kDa |
| 翻译后修饰 | Acetylation; Phosphoprotein. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG45477 anti-DNA polymerase alpha / POLA antibody ICC/IF image
Immunofluorescence: Hela stained with ARG45477 anti-DNA polymerase alpha / POLA antibody at 5 μg/ml dilution.
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ARG45477 anti-DNA polymerase alpha/ POLA antibody WB image
Western blot: HEK293 stained with ARG45477 anti-DNA polymerase alpha / POLA antibody at 0.5 μg/ml dilution.
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ARG45477 anti-DNA polymerase alpha / POLA antibody FACS image
Flow Cytometry: U87 stained with ARG45477 anti-DNA polymerase alpha / POLA antibody at 1 µg/10^6 cells dilution.
