ARG40038
anti-DNA Ligase 3 antibody
anti-DNA Ligase 3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DNA Ligase 3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DNA Ligase 3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 730-1009 of Human DNA Ligase 3 (NP_039269.2). |
| 偶联标记 | Un-conjugated |
| 別名 | DNA ligase III; EC 6.5.1.1; Polydeoxyribonucleotide synthase [ATP] 3; LIG2; DNA ligase 3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 112 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LIG3 |
| 全名 | ligase III, DNA, ATP-dependent |
| 背景介绍 | This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair. [UniProt] |
| 细胞定位 | Isoform 1: Mitochondrion. Note=Contains an N-terminal mitochondrial transit peptide. Isoform 2: Mitochondrion. Note=Contains an N-terminal mitochondrial transit peptide. Isoform 3: Nucleus. Note=Lacks the N-terminal mitochondrial transit peptide. Isoform 4: Nucleus. Note=Lacks the N-terminal mitochondrial transit peptide. [UniProt] |
| 预测分子量 | 113 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG40038 anti-DNA Ligase 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oophoroma stained with ARG40038 anti-DNA Ligase 3 antibody at 1:100 dilution.
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ARG40038 anti-DNA Ligase 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human esophageal cancer stained with ARG40038 anti-DNA Ligase 3 antibody at 1:100 dilution.
