ARG10650
anti-DMPK antibody
anti-DMPK antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DMPK |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Gpig, Hrs |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DMPK |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the center region of Human DMPK. |
| 偶联标记 | Un-conjugated |
| 別名 | Myotonin-protein kinase; DM; MDPK; DM1 protein kinase; MT-PK; DMK; DM-kinase; DM1; DMPK; EC 2.7.11.1; Myotonic dystrophy protein kinase; DM1PK |
应用说明
| 预测反应性说明 | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rat: 100% | ||||||
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| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.09% (w/v) Sodium azide and 2% Sucrose. |
| 抗菌剂 | 0.09% (w/v) Sodium azide |
| 稳定剂 | 2% Sucrose |
| 浓度 | 0.5 - 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DMPK |
| 全名 | dystrophia myotonica-protein kinase |
| 背景介绍 | The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity. [UniProt] |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Phosphorylated. Autophosphorylates. Phosphorylation by RAF1 may result in activation of DMPK. Proteolytic processing of the C-terminus may remove the transmembrane domain and release the kinase from membranes stimulating its activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG10650 anti-DMPK antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue stained with ARG10650 anti-DMPK antibody at 5 µg/ml dilution.
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ARG10650 anti-DMPK antibody WB image
Western blot: Human fetal muscle lysate stained with ARG10650 anti-DMPK antibody at 1 µg/ml dilution.
