ARG64688

Name: anti-DMP1 antibody
Brand: arigo Biolaboratories
SKU: ARG64688
Price: 499 USD
Availability: InStock
Rating: 4.0 (1 reviews)

anti-DMP1 antibody

Datasheet

参考文献3

订购信息

包装

价格 (USD)

100 μg

499

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Goat Polyclonal antibody recognizes DMP1
反应物种	Hu
应用	ICC/IF, WB
特异性	This antibody is expected to recognize both reported isoforms (NP_004398.1; NP_001073380.1).
宿主	Goat
克隆	Polyclonal
同位型	IgG
靶点名称	DMP1
抗原物种	Human
抗原	C-ENSEQANTQDSG
偶联标记	Un-conjugated
別名	DMP-1; Dentin matrix protein 1; ARHP; Dentin matrix acidic phosphoprotein 1; ARHR

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:200
WB	1 - 3 µg/ml

应用说明

WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from goat serum by antigen affinity chromatography.
缓冲液	Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂	0.02% Sodium azide
稳定剂	0.5% BSA
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 1758 Human DMP1 Swiss-port # Q13316 Human Dentin matrix acidic phosphoprotein 1
背景介绍	Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
研究领域	Developmental Biology antibody; Signaling Transduction antibody
预测分子量	56 kDa
翻译后修饰	Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif.

检测图片 (3) Click the Picture to Zoom In

ARG64688 anti-NGEF Antibody WB image

Western blot: human dental pulp stem cells (hDPSCs) stained with ARG64688 anti-NGEF Antibody.

From Kim Hong-Bae et al. Sci Rep. (2018), doi: 10.1038/s41598-018-19395-x, Fig. 5C.
ARG64688 anti-DMP1 antibody ICC/IF image

Immunofluorescence: WJMSC cells fixed with 10% Formalin for 20 min and blocked with PBS, 5% FBS and 0.1% Triton at RT for 30 min. Cells were incubated in PBS with 5% FBS and stained with ARG64688 anti-DMP1 antibody (green) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
ARG64688 anti-DMP1 antibody WB image

Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG64688 anti-DMP1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.