ARG23665
anti-DLL1 antibody [HMD1-5] (PE)
anti-DLL1 antibody [HMD1-5] (PE) for Flow cytometry and Mouse,Rat
概述
| 产品描述 | PE-conjugated Hamster Monoclonal antibody [HMD1-5] recognizes DLL1. This product specifically recognizes Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signaling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4.The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL1 is widely expressed, and acts as a mediator of cell fate decisions during hematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signaling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB). Hamster anti Mouse Delta-Like Protein 1 antibody, clone HMD1-5 blocks binding of Notch2 to Dll1 (Moriyama et al. 2008) |
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| 反应物种 | Ms, Rat |
| 应用 | FACS |
| 宿主 | Hamster |
| 克隆 | Monoclonal |
| 克隆号 | HMD1-5 |
| 同位型 | IgG |
| 靶点名称 | DLL1 |
| 抗原物种 | Mouse |
| 抗原 | DLL1-expressing CHO cells. |
| 偶联标记 | PE |
| 別名 | DELTA1; H-Delta-1; Drosophila Delta homolog 1; Delta-like protein 1; DL1; Delta1; Delta |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DLL1 |
| 全名 | delta-like 1 (Drosophila) |
| 背景介绍 | DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. [UniProt] |
| 预测分子量 | 78 kDa |
| 翻译后修饰 | Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation (By similarity). Ubiquitinated; promotes recycling back to the plasma membrane and confers a strong affinity for NOTCH1. Multi-ubiquitination of LYS-613 by MIB1 promotes both cis and trans-interaction with NOTCH1, as well as activation of Notch signaling. Ubiquitinated by NEURL1B (By similarity). Phosphorylated in a membrane association-dependent manner. Phosphorylation at Ser-697 requires the presence of Ser-694, whereas phosphorylation at Ser-694 occurs independently of the other site. Phosphorylation is required for full ligand activity in vitro and affects surface presentation, ectodomain shedding, and endocytosis. O-fucosylated. Can be elongated to a disaccharide by MFNG. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG23665 anti-DLL1 antibody [HMD1-5] (PE) FACS image
Flow Cytometry: Mouse Delta like protein 1 transfected cells stained with ARG23665 anti-DLL1 antibody [HMD1-5] (PE).
