ARG58554
anti-DIAPH2 antibody
anti-DIAPH2 antibody for Western blot and Human,Mouse,Rat,Rabbit
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DIAPH2 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Rb |
| 应用 | WB |
| 特异性 | This antibody was affinity purified using DIAPH2 (C-terminal region) peptide (without carrier). The antibody detects a 135 kDa protein corresponding to the apparent molecular mass of DIAPH2 on SDS-PAGE immunoblots of various cells and tissues, including human A431 and HeLa cells, mouse brain tissue and C2C12 cells, rat PC12 cells, and rabbit spleen fibroblasts. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DIAPH2 |
| 抗原物种 | Mouse |
| 抗原 | KLH-conjugated synthetic peptide around the C-terminal region of Mouse DIAPH2. This peptide sequence is highly conserved in rat and human mDia2, and has low homology to other formins. |
| 偶联标记 | Un-conjugated |
| 別名 | Diaphanous-related formin-2; DRF2; Protein diaphanous homolog 2; POF2; DIA; POF; DIA2 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 135 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DIAPH2 |
| 全名 | diaphanous-related formin 2 |
| 背景介绍 | The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton. [UniProt] |
| 预测分子量 | 126 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG58554 anti-DIAPH2 antibody WB image
Western blot: PC12, A431, Mouse brain and Rabbit spleen fibroblast lysates stained with ARG58554 anti-DIAPH2 antibody at 1:500 dilution.
