ARG55361
anti-DHX9 antibody
anti-DHX9 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DHX9 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Bov |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DHX9 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human DHX9. |
| 偶联标记 | Un-conjugated |
| 別名 | NDHII; Nuclear DNA helicase II; Leukophysin; ATP-dependent RNA helicase A; NDH2; NDH II; EC 3.6.4.13; RHA; DEAH box protein 9; DDX9; LKP |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | 293 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DHX9 |
| 全名 | DEAH (Asp-Glu-Ala-His) box helicase 9 |
| 背景介绍 | This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010] |
| 生物功能 | Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. Positively regulates HIV-1 LTR-directed gene expression. [UniProt] |
| 细胞定位 | Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 141 kDa |
| 翻译后修饰 | Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization. May be phosphorylated by PRKDC/XRCC7. Phosphorylated by EIF2AK2/PKR and this phosphorylation perturbs its association with dsRNA. |
检测图片 (3) Click the Picture to Zoom In
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ARG55361 anti-DHX9 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG55361 anti-DHX9 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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ARG55361 anti-DHX9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon section stained with ARG55361 anti-DHX9 antibody at 1:25 dilution.
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ARG55361 anti-DHX9 antibody WB image
Western blot: 35 µg of 293 cell lysate stained with ARG55361 anti-DHX9 antibody.
