ARG43427
anti-DHODH antibody
anti-DHODH antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DHODH |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DHODH |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 132-173 of Human DHODH. (RVFRL PEDQA VINRY GFNSH GLSVV EHRLR ARQQK QAKLT ED) |
| 偶联标记 | Un-conjugated |
| 別名 | URA1; POADS; DHOdehase; Dihydroorotate dehydrogenase (quinone), mitochondrial; DHOdehase; EC 1.3.5.2; Dihydroorotate oxidase |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 43 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DHODH |
| 全名 | dihydroorotate dehydrogenase (quinone) |
| 背景介绍 | The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. [UniProt] |
| 细胞定位 | Mitochondrion inner membrane; Single-pass membrane protein. [UniProt] |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG43427 anti-DHODH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43427 anti-DHODH antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43427 anti-DHODH antibody WB image
Western blot: 30 µg of sample under reducing conditions. Rat liver, Mouse spleen and HepG2 whole cell lysates stained with ARG43427 anti-DHODH antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43427 anti-DHODH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43427 anti-DHODH antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43427 anti-DHODH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43427 anti-DHODH antibody at 1 µg/ml dilution, overnight at 4°C.
