ARG56151
anti-DHCR7 antibody
anti-DHCR7 antibody for ICC/IF,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DHCR7 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DHCR7 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 437-463 (C-terminus) of Human DHCR7. |
| 偶联标记 | Un-conjugated |
| 別名 | 7-DHC reductase; SLOS; EC 1.3.1.21; 7-dehydrocholesterol reductase; Putative sterol reductase SR-2; Sterol Delta |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HepG2 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DHCR7 |
| 全名 | 7-dehydrocholesterol reductase |
| 背景介绍 | This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009] |
| 生物功能 | Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC). [UniProt] |
| 预测分子量 | 54 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG56151 anti-DHCR7 antibody ICC/IF image
Immunofluorescence: 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells stained with ARG56151 anti-DHCR7 antibody (green) at 1:25 dilution. Cytoplasmic actin is stained with Dylight® 554 conjugated with Phalloidin (red) at 1:100 dilution. DAPI (blue) for nuclear staining.
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ARG56151 anti-DHCR7 antibody WB image
Western blot: 20 µg of HepG2 cell lysate stained with ARG56151 anti-DHCR7 antibody at 1:2000 dilution.
