ARG45441
anti-DGCR8 antibody
anti-DGCR8 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DGCR8 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DGCR8 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human DGCR8. |
| 偶联标记 | Un-conjugated |
| 別名 | DGCR8; DGCR8 Microprocessor Complex Subunit; DGCRK6; C22orf12; Pasha; Gy1; DiGeorge Syndrome Critical Region Gene 8; Microprocessor Complex Subunit DGCR8; DiGeorge Syndrome Critical Region 8; DGCR8, Microprocessor Complex Subunit; Chromosome 22 Open Reading Frame 12 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8WYQ5 Human Microprocessor complex subunit DGCR8 Swiss-port # Q9EQM6 Mouse Microprocessor complex subunit DGCR8 |
|---|---|
| 基因名称 | DGCR8 |
| 全名 | DGCR8 Microprocessor Complex Subunit |
| 背景介绍 | This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| 生物功能 | Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 86 kDa |
| 翻译后修饰 | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG45441 anti-DGCR8 antibody IHC-P image
Immunohistochemistry: Human rectal cancer stained with ARG45441 anti-DGCR8 antibody at 1 μg/ml dilution.
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ARG45441 anti-DGCR8 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45441 anti-DGCR8 antibody at 2 μg/ml dilution.
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ARG45441 anti-DGCR8 antibody WB image
Western blot: Hela and human placenta stained with ARG45441 anti-DGCR8 antibody at 0.5 μg/ml dilution.
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ARG45441 anti-DGCR8 antibody IHC-P image
Immunohistochemistry: Mouse small intestine stained with ARG45441 anti-DGCR8 antibody at 1 μg/ml dilution.
