ARG54370
anti-DEDAF antibody
anti-DEDAF antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DEDAF |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 特异性 | This antibody recognizes human, mouse, and rat DEDAF (32kDa). |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DEDAF |
| 抗原物种 | Human |
| 抗原 | Peptide corresponding to aa 215-229 of human DEDAF (accession no. AF179286). |
| 偶联标记 | Un-conjugated |
| 別名 | YEAF1; RING1 and YY1-binding protein; DED-associated factor; Death effector domain-associated factor; YY1 and E4TF1-associated factor 1; AAP1; Apoptin-associating protein 1; DEDAF; APAP-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | A549 and HepG2 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunoaffinity chroma-tography |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RYBP |
| 全名 | RING1 and YY1 binding protein |
| 背景介绍 | Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. A novel protein that interacts with the death effector domain of capase-8 and caspase-10 has been designated DEDAF and is dedentical to the transcriptional repressor RYBP. DEDAF is expressed in multiple tissues and cell lines and appears to be involved in the regulation of both cytoplasmic and nuclear events of apoptosis. |
| 生物功能 | Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Monoubiquitinated. |
检测图片 (3) Click the Picture to Zoom In
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ARG54370 anti-DEDAF antibody WB image
Western Blot: A:A549; B:HepG2; C:mouse 3T3 stained with ARG54370 anti-DEDAF antibody at 1 μg/ml dilution.
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ARG54370 anti-DEDAF antibody IHC-P image
Immunohistochemistry: mouse liver stained with ARG54370 anti-DEDAF antibody at 20 μg/ml dilution.
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ARG54370 anti-DEDAF antibody IHC-P image
Immunohistochemistry: mouse liver stained with ARG54370 anti-DEDAF antibody at 5 μg/ml dilution.
