ARG63911
anti-DDX6 antibody
anti-DDX6 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes DDX6 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DDX6 |
| 抗原物种 | Human |
| 抗原 | C-AEYHSEPVEDEKP |
| 偶联标记 | Un-conjugated |
| 別名 | Probable ATP-dependent RNA helicase DDX6; DEAD box protein 6; Oncogene RCK; ATP-dependent RNA helicase p54; EC 3.6.4.13; RCK; P54; HLR2 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P26196 Human Probable ATP-dependent RNA helicase DDX6 |
|---|---|
| 基因名称 | DDX6 |
| 全名 | DEAD-box helicase 6 |
| 背景介绍 | This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012] |
| 生物功能 | In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573). [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Sumoylated (PubMed:26184334). |
检测图片 (4) Click the Picture to Zoom In
-
ARG63911 anti-DDX6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63911 anti-DDX6 antibody at 5 µg/ml dilution followed by AP-staining.
-
ARG63911 anti-DDX6 antibody WB image
Western blot: 35 µg of Daudi (A), Jurkat (B) and HepG2 (C) lysates (in RIPA buffer) stained with ARG63911 anti-DDX6 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
-
ARG63911 anti-DDX6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thymus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63911 anti-DDX6 antibody at 5 µg/ml dilution followed by AP-staining.
-
ARG63911 anti-DDX6 antibody WB image
Western blot: 1) Mock transfection and 2) Human DDX6 (RC209431) expressing plasmid transfected HEK293 cell lysate stained with ARG63911 anti-DDX6 antibody.
