ARG41744

anti-DDX6 antibody

anti-DDX6 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes DDX6
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 DDX6
抗原物种 Human
抗原 Synthetic peptide within aa. 400 to the C-terminus of Human DDX6 (NP_001244120.1).
偶联标记 Un-conjugated
別名 Probable ATP-dependent RNA helicase DDX6; DEAD box protein 6; Oncogene RCK; ATP-dependent RNA helicase p54; EC 3.6.4.13; RCK; P54; HLR2

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Rat spleen
实际分子量 ~ 54 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 13209 Mouse DDX6

GeneID: 1656 Human DDX6

Swiss-port # P26196 Human Probable ATP-dependent RNA helicase DDX6

Swiss-port # P54823 Mouse Probable ATP-dependent RNA helicase DDX6

基因名称 DDX6
全名 DEAD (Asp-Glu-Ala-Asp) box helicase 6
背景介绍 This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012]
生物功能 In the process of mRNA degradation, may play a role in mRNA decapping. [UniProt]
细胞定位 Cytoplasm, P-body. Cytoplasm. Nucleus. Note=Upon cellular stress, relocalizes to stress granules (PubMed:26184334). [UniProt]
预测分子量 54 kDa
翻译后修饰 Sumoylated (PubMed:26184334). [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG41744 anti-DDX6 antibody ICC/IF image

    Immunofluorescence: L929 cells stained with ARG41744 anti-DDX6 antibody at 1:100 dilution.

  • ARG41744 anti-DDX6 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG41744 anti-DDX6 antibody at 1:100 dilution.

  • ARG41744 anti-DDX6 antibody WB image

    Western blot: 25 µg of Rat spleen lysate stained with ARG41744 anti-DDX6 antibody at 1:1000 dilution.