ARG63435
anti-DDX5 / p68 RNA helicase antibody
anti-DDX5 / p68 RNA helicase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes DDX5 / p68 RNA helicase |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Dog, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DDX5 / p68 RNA helicase |
| 抗原物种 | Human |
| 抗原 | C-PMIGYPMPTGYSQ |
| 偶联标记 | Un-conjugated |
| 別名 | Probable ATP-dependent RNA helicase DDX5; DEAD box protein 5; p68; HUMP68; EC 3.6.4.13; G17P1; RNA helicase p68; HLR1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P17844 Human Probable ATP-dependent RNA helicase DDX5 |
|---|---|
| 背景介绍 | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Arg-502 is dimethylated, probably to asymmetric dimethylarginine. Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination. Polyubiquitinated, leading to proteasomal degradation. |
检测图片 (4) Click the Picture to Zoom In
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ARG63435 anti-DDX5 / p68 RNA helicase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63435 anti-DDX5 / p68 RNA helicase antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63435 anti-DDX5 / p68 RNA helicase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63435 anti-DDX5 / p68 RNA helicase antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63435 anti-DDX5 / p68 RNA helicase antibody WB image
Western blot: 35 µg of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and NIH/3T3 (E) cell lysates (in RIPA buffer) stained with ARG63435 anti-DDX5 / p68 RNA helicase antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
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ARG63435 anti-DDX5 / p68 RNA helicase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63435 anti-DDX5 / p68 RNA helicase antibody at 2.5 µg/ml dilution followed by AP-staining.
客户反馈
Good
anti-DDX5 / p68 RNA helicase antibody
Application：WB
Sample：293T and HeLa cells
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
