ARG58121

anti-DDX4 antibody

anti-DDX4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes DDX4
反应物种 Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 DDX4
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 1-300 of Human DDX4 (NP_077726.1).
偶联标记 Un-conjugated
別名 Probable ATP-dependent RNA helicase DDX4; VASA; Vasa homolog; DEAD box protein 4; EC 3.6.4.13; MVH; Mouse Vasa Homologue

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:200 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Mouse testis
实际分子量 ~ 79 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 13206 Mouse DDX4

GeneID: 310090 Rat DDX4

Swiss-port # Q61496 Mouse Probable ATP-dependent RNA helicase DDX4

Swiss-port # Q64060 Rat Probable ATP-dependent RNA helicase DDX4

基因名称 DDX4
全名 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
背景介绍 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
生物功能 May play a role in germ cell development. May play a role in sperm motility. [UniProt]
细胞定位 Cytoplasm, perinuclear region. Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. [UniProt]
预测分子量 79 kDa

检测图片 (1) Click the Picture to Zoom In

  • ARG58121 anti-DDX4 antibody WB image

    Western blot: 25 µg of Mouse testis lysate stained with ARG58121 anti-DDX4 antibody at 1:1000 dilution.