ARG45471

anti-DDX21 antibody

anti-DDX21 antibody for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes DDX21
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 DDX21
抗原物种 Human
抗原 Recombinant protein containing to human DDX21.
偶联标记 Un-conjugated
別名 DDX21; DExD-Box Helicase 21; Gu-Alpha; Nucleolar RNA Helicase 2; RH-II/GU; GURDB; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21; Nucleolar RNA Helicase Gu; Nucleolar RNA Helicase II; DEAD-Box Helicase 21; DEAD Box Protein 21; RH II/Gu; DEAD (Asp-Glu-Ala-Asp) Box Helicase 21; RNA Helicase II/Gu Alpha; EC 3.6.4.13; Gu Protein; RH-II/GuA; EC 3.6.1 47; II/Gu; GUA; RH

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 86 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 9188 Human DDX21

Swiss-port # Q9NR30 Human Nucleolar RNA helicase 2

基因名称 DDX21
全名 DExD-Box Helicase 21
背景介绍 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. [provided by RefSeq, Jul 2008]
生物功能 RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II) [UniProt]
细胞定位 Cytoplasm; Mitochondrion; Nucleus. [UniProt]
预测分子量 87 kDa
翻译后修饰 Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG45471 anti-DDX21 antibody ICC/IF image

    Immunofluorescence: Caco-2 stained with ARG45471 anti-DDX21 antibody at 5 μg/ml dilution.

  • ARG45471 anti-DDX21 antibody WB image

    Western blot: HEK293 stained with ARG45471 anti-DDX21 antibody at 0.5 μg/ml dilution.

  • ARG45471 anti-DDX21 antibody FACS image

    Flow Cytometry: A431 stained with ARG45471 anti-DDX21 antibody at 1 µg/10^6 cells dilution.