ARG62342
anti-DDDDK tag antibody [FG4R]
anti-DDDDK tag antibody [FG4R] for Dot blot,ELISA,ICC/IF,Immunoprecipitation,In situ hybridization,Western blot and Other
Controls and Markers antibody; Tag Internal Control antibody
参考文献5
概述
| 产品描述 | Mouse Monoclonal antibody [FG4R] recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies). |
|---|---|
| 反应物种 | Other |
| 应用 | Dot, ELISA, ICC/IF, IP, ISH, WB |
| 特异性 | Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | FG4R |
| 同位型 | IgG1 |
| 靶点名称 | DDDDK tag |
| 抗原物种 | Others |
| 抗原 | DYKDDDDK (FLAG) synthetic peptide conjugated to KLH. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A Purified |
| 纯化说明 | Protein A affinity chromatography from mouse ascites fluid. |
| 缓冲液 | 10mM PBS (pH 7.2) and 0.05% Sodium azide. |
| 抗菌剂 | 0.05% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 全名 | DDDDK Epitope Tag |
|---|---|
| 背景介绍 | Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal xxxDDDDK from a fusion protein has been reported (Humphreys, D.P., et al., 1999). A sequence motif with five out of eight amino acid residues identical to the xxxDDDDK is found in both rat and mouse Mg2+ dependent protein b-phosphatase, as well as in the human and bovine enzyme. |
| 产品亮点 | Related products: DDDDK antibodies; DDDDK Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Controls and Markers antibody; Tag Internal Control antibody |
检测图片 (1) Click the Picture to Zoom In
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ARG62342 anti-DDDDK antibody [FG4R] WB image
Western blot: 20 µg of 293T and 293T+flag+mCherry cell lysates stained with ARG62342 anti-DDDDK antibody [FG4R] at 1:1000 dilution.
客户反馈
Excellent
anti-DDDDK tag antibody [FG4R]
Application：WB
Sample：293T and 293T+flag+mCherry
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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