ARG63154
anti-DDB1 antibody
anti-DDB1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes DDB1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DDB1 |
| 抗原物种 | Human |
| 抗原 | C-DLIKVVEELTRIH |
| 偶联标记 | Un-conjugated |
| 別名 | XPCE; XPCe; DDB p127 subunit; DDBa; UV-damaged DNA-binding factor; HBV X-associated protein 1; DDBA; UV-damaged DNA-binding protein 1; XPE; XAP-1; Damage-specific DNA-binding protein 1; XPE-BF; DNA damage-binding protein a; UV-DDB1; UV-DDB 1; XAP1; Xeroderma pigmentosum group E-complementing protein; XPE-binding factor; DNA damage-binding protein 1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. [provided by RefSeq, May 2012] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 127 kDa |
| 翻译后修饰 | Phosphorylated by ABL1. Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. |
检测图片 (4) Click the Picture to Zoom In
-
ARG63154 anti-DDB1 antibody WB image
Western Blot: NSO lysate (1E5 cells per lane) stained with ARG63154 anti-DDB1 antibody at 1 µg/ml dilution.
-
ARG63154 anti-DDB1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63154 anti-DDB1 antibody at 5 µg/ml dilution followed by AP-staining.
-
ARG63154 anti-DDB1 antibody WB image
Western blot: 35 µg of HeLa (A), HepG2 (B) and Jurkat (C) cell lysates (in RIPA buffer) stained with ARG63154 anti-DDB1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
-
ARG63154 anti-DDB1 antibody WB image
Western blot: 35 µg of NIH/3T3 (A) and NSO (B) cell lysates (in RIPA buffer) stained with ARG63154 anti-DDB1 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
