ARG65153
anti-DCUN1D1 antibody
anti-DCUN1D1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes DCUN1D1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Cow |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DCUN1D1 |
| 抗原物种 | Human |
| 抗原 | C-RPQIAGTKSTT |
| 偶联标记 | Un-conjugated |
| 別名 | SCRO; RP42; Defective in cullin neddylation protein 1-like protein 1; Squamous cell carcinoma-related oncogene; DCUN1 domain-containing protein 1; SCCRO; DCN1-like protein 1; DCUN1L1; DCNL1; Tes3 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DCUN1D1 |
| 全名 | DCN1, defective in cullin neddylation 1, domain containing 1 |
| 生物功能 | Part of an E3 ubiquitin ligase complex for neddylation. Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates (PubMed:25349211). Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity). [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody |
| 预测分子量 | 30 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG65153 anti-DCUN1D1 antibody WB image
Western Blot: HepG2 lysate (35 µg protein in RIPA buffer) stained with ARG65153 anti-DCUN1D1 antibody at 0.5 µg/ml dilution.
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ARG65153 anti-DCUN1D1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65153 anti-DCUN1D1 antibody at 5 µg/ml dilution followed by AP-staining.
