ARG53991
anti-DCTN1 / p150-glued antibody (N-term)
anti-DCTN1 / p150-glued antibody (N-term) for Immunoprecipitation,Western blot and Human
Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes DCTN1 / p150-glued |
|---|---|
| 反应物种 | Hu |
| 应用 | IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | DCTN1 / p150-glued |
| 抗原物种 | Human |
| 抗原 | E.coli expressed purified recombinant DCTN1 / p150-glued protein fragments corresponding to 1-150 a.a of human Dynactin subunit 1 protein p150 isoform. |
| 偶联标记 | Un-conjugated |
| 別名 | P135; p150-glued; p135; DP-150; Dynactin subunit 1; 150 kDa dynein-associated polypeptide; DAP-150 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 150 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DCTN1 |
| 全名 | dynactin 1 |
| 背景介绍 | Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. |
| 生物功能 | Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. [UniProt] |
| 细胞定位 | Cytoplasm |
| 研究领域 | Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 142 kDa |
| 翻译后修饰 | Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. Phosphorylation by SLK at Thr-145, Thr-146 and Thr-147 targets DCTN1 to the centrosome. It is uncertain if SLK phosphorylates all three threonines or one or two of them. PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope, promotes its dissociation from microtubules during early mitosis and positively regulates nuclear envelope breakdown during prophase. |
检测图片 (2) Click the Picture to Zoom In
-
ARG53991 anti-DCTN1 / p150-glued antibody (N-term) WB image
Western blot: 293T cell lysate stained with ARG53991 anti-DCTN1 / p150-glued antibody (N-term) at 1:500 dilution.
-
ARG53991 anti-DCTN1 / p150-glued antibody (N-term) IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG53991 anti-DCTN1 / p150-glued antibody (N-term).
