ARG58518
anti-DCC antibody
anti-DCC antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DCC |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Hm |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DCC |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence at the N-terminus of Human DCC(162-178aa EVIGEPMPTIHWQKNQQ), identical to the related Mouse sequence, and different from the related Rat sequence by one amino acid. |
| 偶联标记 | Un-conjugated |
| 別名 | Tumor suppressor protein DCC; Immunoglobulin superfamily DCC subclass member 1; CRC18; NTN1R1; MRMV1; Colorectal cancer suppressor; IGDCC1; CRCR1; Netrin receptor DCC |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DCC |
| 全名 | DCC netrin 1 receptor |
| 背景介绍 | This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma. [provided by RefSeq, Oct 2009] |
| 生物功能 | Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene. [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 158 kDa |
| 翻译后修饰 | Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58518 anti-DCC antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer stained with ARG58518 anti-DCC antibody.
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ARG58518 anti-DCC antibody WB image
Western blot: U87, SW620, COLO320, 293T and HeLa cell lysates stained with ARG58518 anti-DCC antibody.
