ARG46553
anti-DCAF12 antibody
anti-DCAF12 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Chicken Polyclonal antibody recognizes DCAF12 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Chicken |
| 克隆 | Polyclonal |
| 同位型 | IgY |
| 靶点名称 | DCAF12 |
| 抗原物种 | Human |
| 抗原 | A 15 amino acid synthetic peptide within the last 50 amino acids of human DCAF12. |
| 偶联标记 | Un-conjugated |
| 別名 | DCAF12; DDB1 and CUL4 associated factor 12; TCC52; CT102; TCC52; WDR40A; KIAA1892; DDB1- and CUL4-associated factor 12; Centrosome-related protein TCC52 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity chromatography purified |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -603°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q5T6F0 Human DDB1- and CUL4-associated factor 12 Swiss-port # Q8BGZ3 Mouse DDB1- and CUL4-associated factor 12 |
|---|---|
| 基因名称 | DCAF12 |
| 全名 | DDB1 and CUL4 associated factor 12 |
| 背景介绍 | This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. [provided by RefSeq, Jul 2009] |
| 生物功能 | Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:16949367, PubMed:16964240, PubMed:29779948). The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:29779948). The DCX(DCAF12) complex specifically recognizes proteins with a diglutamate (Glu-Glu) at the C-terminus, such as MAGEA3, MAGEA6 and CCT5, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:31267705). Ubiquitination of MAGEA3, MAGEA6 by DCX(DCAF12) complex is required for starvation-induced autophagy (PubMed:31267705). Also directly recognizes the C-terminal glutamate-leucine (Glu-Leu) degron as an alternative degron in proteins such as MOV10, leading to their ubiquitination and degradation. Controls the protein level of MOV10 during spermatogenesis and in T cells, especially after their activation (PubMed:34065512). [UniProt] |
| 细胞定位 | Cytoplasm; Nucleus; Cytoskeleton. [UniProt] |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Phosphoprotein; Ubl conjugation. [UniProt] |
