ARG45612
anti-DBT antibody
anti-DBT antibody for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DBT |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DBT |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human DBT. |
| 偶联标记 | Un-conjugated |
| 別名 | DBT; BCATE2; Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial; EC 2.3.1.168; 52 kDa mitochondrial autoantigen of primary biliary cirrhosis; Branched chain 2-oxo-acid dehydrogenase complex component E2; BCOADC-E2; Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue; 2-methylpropanoyltransferase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P11182 Human Lipoamide acyltransferase component of branched-chain alpha-keto acid dehy Swiss-port # P53395 Mouse Lipoamide acyltransferase component of branched-chain alpha-keto acid dehy |
|---|---|
| 基因名称 | DBT |
| 全名 | Dihydrolipoamide Branched Chain Transacylase E2 |
| 背景介绍 | The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. [UniProt] |
| 细胞定位 | Mitochondrion. [UniProt] |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Acetylation; Phosphoprotein. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG45612 anti-DBT antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG45612 anti-DBT antibody at 5 μg/ml dilution.
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ARG45612 anti-DBT antibody WB image
Western blot: SiHa and A431 stained with ARG45612 anti-DBT antibody at 0.5 μg/ml dilution.
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ARG45612 anti-DBT antibody IP image
Immunoprecipitation: A431 lysate immunoprecipitated with 2 μg ARG45612 anti-DBT antibody.
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ARG45612 anti-DBT antibody FACS image
Flow Cytometry: SiHa stained with ARG45612 anti-DBT antibody at 1 µg/10^6 cells dilution.
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ARG45612 anti-DBT antibody WB image
Western blot: Rat stomach stained with ARG45612 anti-DBT antibody at 0.5 μg/ml dilution.
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ARG45612 anti-DBT antibody WB image
Western blot: Mouse stomach stained with ARG45612 anti-DBT antibody at 0.5 μg/ml dilution.
