ARG45814

anti-DBF4 antibody

anti-DBF4 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes DBF4
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 DBF4
抗原物种 Human
抗原 Recombinant protein containing to human DBF4.
偶联标记 Un-conjugated
別名 DBF4; DBF4-CDC7 Kinase Regulatory Subunit; ZDBF1; DBF4A; ASK; Activator Of S Phase Kinase; Chif; DBF4-Type Zinc Finger-Containing Protein 1; Zinc Finger, DBF-Type Containing 1; Regulatory Subunit Of DDK; Protein DBF4 Homolog A; DBF4 Zinc Finger A; Chiffon Homolog A; Chiffon Homolog (Drosophila); DBF4 Homolog (S. Cerevisiae); DBF4 Zinc Finger; DBF4 Homolog

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 80 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10926 Human DBF4

GeneID: 27214 Mouse DBF4

Swiss-port # Q9QZ41 Mouse Protein DBF4 homolog A

Swiss-port # Q9UBU7 Human Protein DBF4 homolog A

基因名称 DBF4
全名 DBF4-CDC7 Kinase Regulatory Subunit
背景介绍 Predicted to enable protein serine/threonine kinase activator activity. Predicted to be involved in positive regulation of nuclear cell cycle DNA replication and regulation of cell cycle phase transition. Located in nuclear body. [provided by Alliance of Genome Resources, Feb 2025]
生物功能 Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 77 kDa
翻译后修饰 Phosphoprotein. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG45814 anti-DBF4 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG45814 anti-DBF4 antibody at 5 μg/ml dilution.

  • ARG45814 anti-DBF4 antibody WB image

    Western blot: HepG2 stained with ARG45814 anti-DBF4 antibody at 0.5 μg/ml dilution.

  • ARG45814 anti-DBF4 antibody FACS image

    Flow Cytometry: Raji stained with ARG45814 anti-DBF4 antibody at 1 µg/10^6 cells dilution.

  • ARG45814 anti-DBF4 antibody WB image

    Western blot: PC-12 stained with ARG45814 anti-DBF4 antibody at 0.5 μg/ml dilution.

  • ARG45814 anti-DBF4 antibody WB image

    Western blot: NIH/3T3 stained with ARG45814 anti-DBF4 antibody at 0.5 μg/ml dilution.