ARG52256
anti-DARPP32 phospho (Thr75) antibody
anti-DARPP32 phospho (Thr75) antibody for Western blot and Rat
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DARPP32 phospho (Thr75) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Chk, Dog, NHuPrm |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DARPP32 |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | Dopamine- and cAMP-regulated neuronal phosphoprotein; DARPP32; DARPP-32; Protein phosphatase 1 regulatory subunit 1B |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | Specific for the ~32k DARPP-32 protein phosphorylated at Thr75. Immunolabeling is blocked by λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q6J4I0 Rat Protein phosphatase 1 regulatory subunit 1B |
|---|---|
| 基因名称 | PPP1R1B |
| 全名 | protein phosphatase 1, regulatory (inhibitor) subunit 1B |
| 背景介绍 | DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002). |
| 研究领域 | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 23 kDa |
| 翻译后修饰 | Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Phosphorylation of Thr-34 is required for activity. |
检测图片 (1) Click the Picture to Zoom In
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ARG52256 anti-DARPP32 phospho (Thr75) antibody WB image
Western blot: Rat caudate lysate showing phospho-specific immunolabeling of the ~32 kDa DARPP-32 protein phosphorylated at Thr 75 stained with ARG52256 anti-DARPP32 phospho (Thr75) antibody.
