ARG63157

anti-DAPK2 antibody

anti-DAPK2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Cell Death antibody

概述

产品描述 Goat Polyclonal antibody recognizes DAPK2
反应物种 Hu, Ms
预测物种 Dog, Rat, Pig
应用 IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 DAPK2
抗原物种 Human
抗原 C-KALHPRRRSSTS
偶联标记 Un-conjugated
別名 DRP1; DAP-kinase-related protein 1; DRP-1; DAP kinase 2; EC 2.7.11.1; Death-associated protein kinase 2

应用说明

应用建议
应用 推荐稀释比
IHC-P2.5 µg/ml
WB1 - 3 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 13143 Mouse DAPK2

GeneID: 23604 Human DAPK2

Swiss-port # Q8VDF3 Mouse Death-associated protein kinase 2

Swiss-port # Q9UIK4 Human Death-associated protein kinase 2

背景介绍 This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]
研究领域 Cell Biology and Cellular Response antibody; Cell Death antibody
预测分子量 43 kDa
翻译后修饰 Autophosphorylation at Ser-318 inhibits its catalytic activity. Dephosphorylated at Ser-318 in response to activated Fas and TNF-alpha receptors.

检测图片 (3) Click the Picture to Zoom In

  • ARG63157 anti-DAPK2 antibody WB image

    Western Blot: mouse brain extracts (RIPA buffer, 35 µg total protein per lane) stained with ARG63157 anti-DAPK2 antibody at 2 µg/ml dilution.

  • ARG63157 anti-DAPK2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human thyroid tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63157 anti-DAPK2 antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG63157 anti-DAPK2 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Breast. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63157 anti-DAPK2 antibody at 2.5 µg/ml dilution followed by AP-staining.