ARG58561
anti-DAD1 antibody
anti-DAD1 antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DAD1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DAD1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to 14 aa (C-terminus) of Human DAD1. |
| 偶联标记 | Un-conjugated |
| 別名 | Oligosaccharyl transferase subunit DAD1; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; Defender against cell death 1; EC 2.4.99.18; DAD-1; OST2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HepG2 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Ion exchange chromatography purified. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P61803 Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD |
|---|---|
| 基因名称 | DAD1 |
| 全名 | defender against cell death 1 |
| 背景介绍 | DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008] |
| 生物功能 | Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis (By similarity). [UniProt] |
| 预测分子量 | 12 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG58561 anti-DAD1 antibody IF image
Immunofluorescence: HepG2 cells stained with ARG58561 anti-DAD1 antibody at 10 µg/ml dilution.
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ARG58561 anti-DAD1 antibody WB image
Western blot: HepG2 cell lysate stained with ARG58561 anti-DAD1 antibody at 0.5, 1, and 2 µg/ml dilution.
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ARG58561 anti-DAD1 antibody ICC image
Immunocytochemistry: HepG2 cells stained with ARG58561 anti-DAD1 antibody at 10 µg/ml dilution.
