anti-Cytokeratin 8 antibody [SQab1718]

anti-Cytokeratin 8 antibody [SQab1718] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human




Recombinant Rabbit Monoclonal antibody [SQab1718] recognizes Cytokeratin 8

反应物种 Hu
应用 IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab1718
靶点名称 Cytokeratin 8
抗原物种 Human
抗原 Synthetic peptide around the C-terminus of Cytokeratin 8.
偶联标记 Un-conjugated
別名 Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8


应用 推荐稀释比
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 3856 Human KRT8

Swiss-port # P05787 Human Keratin, type II cytoskeletal 8

基因名称 KRT8
全名 keratin 8, type II
背景介绍 This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
生物功能 Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
产品亮点 Related news:
Cancer Pathology Markers (SQ clones)
预测分子量 54 kDa
翻译后修饰 Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

检测图片 (2) Click the Picture to Zoom In

  • ARG66196 anti-Cytokeratin 8 antibody [SQab1718] WB image

    Western blot: 20 µg of HeLa cell lysate stained with ARG66196 anti-Cytokeratin 8 antibody [SQab1718] at 1:500 dilution, overnight at 4°C.

  • ARG66196 anti-Cytokeratin 8 antibody [SQab1718] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66196 anti-Cytokeratin 8 antibody [SQab1718] at 1:200 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0


nuts_pic      Excellent

anti-Cytokeratin 8 antibody [SQab1718]



Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC