ARG66870
anti-Cytokeratin 7 antibody
anti-Cytokeratin 7 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Cytokeratin 7 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG |
| 靶点名称 | Cytokeratin 7 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human Cytokeratin 7. |
| 偶联标记 | Un-conjugated |
| 別名 | SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | ~ 54 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT7 |
| 全名 | keratin 7, type II |
| 背景介绍 | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Arg-20 is dimethylated, probably to asymmetric dimethylarginine. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG66870 anti-Cytokeratin 7 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with anti-FoxO1 antibody (red) at 1:200 dilution, overnight at 4°C, and co-stained with ARG66870 anti-Cytokeratin 7 antibody (green) at 1:200 dilution, overnight at 4°C.
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ARG66870 anti-Cytokeratin 7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG66870 anti-Cytokeratin 7 antibody (red) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG66870 anti-Cytokeratin 7 antibody WB image
Western blot: HeLa, Mouse kidney and Mouse brain lysates stained with ARG66870 anti-Cytokeratin 7 antibody at 1:2000 dilution.
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ARG66870 anti-Cytokeratin 7 antibody IP image
Immunoprecipitation: 1) Input: HeLa cell lysate, 2) IP product: IP dilute 1:200. Using ARG66870 anti-Cytokeratin 7 antibody.
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ARG66870 anti-Cytokeratin 7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG66870 anti-Cytokeratin 7 antibody at 1:200 dilution.
