anti-Cytokeratin 6 antibody [SQab18113]

anti-Cytokeratin 6 antibody [SQab18113] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human,Mouse,Rat


产品描述 Recombinant Rabbit Monoclonal antibody [SQab18113] recognizes Cytokeratin 6
反应物种 Hu, Ms, Rat
应用 IHC-P, IP, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab18113
同位型 IgG
靶点名称 Cytokeratin 6
抗原物种 Human
抗原 Synthetic peptide around the C-terminus of Human Cytokeratin 6.
偶联标记 Un-conjugated
別名 CK-6A; K6D; Cytokeratin-6A; CK-6D; K6C; K6A; Type-II keratin Kb6; PC3; CK6D; KRT6D; CK6A; CK6C; allergen Hom s 5; Keratin, type II cytoskeletal 6A; Keratin-6A; Cytokeratin-6D; KRT6C


应用 推荐稀释比
IHC-P1:50 - 1:100
WB1:5000 - 1:10000
应用说明 IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 16687 Mouse KRT6A

GeneID: 3853 Human KRT6A

Swiss-port # P02538 Human Keratin, type II cytoskeletal 6A

Swiss-port # P50446 Mouse Keratin, type II cytoskeletal 6A

基因名称 KRT6A
全名 keratin 6A, type II
背景介绍 The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Oct 2014]
生物功能 Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair. [UniProt]
预测分子量 60 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG66386 anti-Cytokeratin 6 antibody [SQab18113] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cervix cancer stained with ARG66386 anti-Cytokeratin 6 antibody [SQab18113] at 1:50 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

  • ARG66386 anti-Cytokeratin 6 antibody [SQab18113] WB image

    Western blot: 10 µg of A431, Mouse skin and Rat skin lysates stained with ARG66386 anti-Cytokeratin 6 antibody [SQab18113] at 1:10000 dilution.

  • ARG66386 anti-Cytokeratin 6 antibody [SQab18113] IP image

    Immunoprecipitation: 0.4 mg of A431 whole cell lysate was immunoprecipitated (1:50 dilution) and stained with ARG66386 anti-Cytokeratin 6 antibody [SQab18113].

    Lane 1: Immunoprecipitation in A431 whole cell lysate
    Lane 2: PBS instead of Primary Ab in A431 whole cell lysate
    Lane 3: A431 whole cell lysate, 10 µg (input)