anti-Cytokeratin 20 antibody [SQab1737]

anti-Cytokeratin 20 antibody [SQab1737] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

CK7/CK20 Carcinoma Study antibody


产品描述 Recombinant Rabbit Monoclonal antibody [SQab1737] recognizes Cytokeratin 20
反应物种 Hu
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab1737
同位型 IgG
靶点名称 Cytokeratin 20
抗原物种 Human
抗原 Synthetic peptide around the C-terminus of Human Cytokeratin 20.
偶联标记 Un-conjugated
別名 KRT21; CK20; K20; CD20; CK-20; Keratin-20; Cytokeratin-20; Keratin, type I cytoskeletal 20; Protein IT


应用 推荐稀释比
FACS1:10 - 1:1000
ICC/IF1:50 - 1:2000
IHC-P1:800 - 1:1600
WB1:2000 - 1:10000
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 54474 Human KRT20

Swiss-port # P35900 Human Keratin, type I cytoskeletal 20

基因名称 KRT20
全名 keratin 20, type I
背景介绍 Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
生物功能 Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30318 CK7 / CK20 Carcinoma Antibody Duo
Related products:
Cytokeratin 20 antibodies; Cytokeratin 20 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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研究领域 CK7/CK20 Carcinoma Study antibody
预测分子量 48 kDa
翻译后修饰 Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).

Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG66248 anti-Cytokeratin 20 antibody [SQab1737] ICC/IF image

    Immunofluorescence: HT-29 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at RT. Samples were stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] (green) at 1:200, 4°C.

  • ARG66248 anti-Cytokeratin 20 antibody [SQab1737] FACS image

    Flow Cytometry: HT-29 cells stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the primary antibody at 1:1000 dilution in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat anti-rabbit Alexa Fluor® 488 (IgG H+L) at 1:2000 dilution for 20 min at 4°C. Unlabelled sample (Blue) was used as a control.

  • ARG66248 anti-Cytokeratin 20 antibody [SQab1737] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded colonic adenocarcinoma stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:800. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG66248 anti-Cytokeratin 20 antibody [SQab1737] WB image

    Western blot: 10 μg of HCT-116 and HT-29 cell lysates stained with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:5000 dilution.

  • ARG66248 anti-Cytokeratin 20 antibody [SQab1737] IP image

    Immunoprecipitation: Cytokeratin 20 was immunoprecipitated from 0.5 mg of HT-29 lysate with ARG66248 anti-Cytokeratin 20 antibody [SQab1737] at 1:50 dilution.

    1. IP by using ARG66248 in HT-29 whole cell lysate
    2. PBS instead of ARG66248 in HT-29 whole cell lysate
    3. 10 µg of HT-29 whole cell lysate (input)