ARG62979
anti-Cytokeratin 19 antibody [A53-B/A2]
anti-Cytokeratin 19 antibody [A53-B/A2] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [A53-B/A2] recognizes Cytokeratin 19 |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, ICC/IF, IHC-P, IP, WB |
| 特异性 | The clone A53-B/A2 reacts with Rod domain of cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | A53-B/A2 |
| 同位型 | IgG2a |
| 靶点名称 | Cytokeratin 19 |
| 抗原物种 | Human |
| 抗原 | MCF-7 human breast adenocarcinoma cell line |
| 偶联标记 | Un-conjugated |
| 別名 | Cytokeratin-19; K19; CK-19; K1CS; Keratin-19; CK19; Keratin, type I cytoskeletal 19 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) or EDTA pH 8 for 10-20 min followed by cooling at RT for 20 min. Or perform enzymatic antigen retrieval with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 10-15 min at or trypsin at 1mg/ml TrisHCl (or PBS), pH 7.4 for 10-15 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT19 |
| 全名 | keratin 19, type I |
| 背景介绍 | Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokeratin 19 involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| 产品亮点 | Related products: Cytokeratin 19 antibodies; Anti-Mouse IgG secondary antibodies; Related news: Therapeutic strategies against PDAC |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 44 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG62979 anti-Cytokeratin 19 antibody [A53-B/A2] WB image
Western blot: 30 µg of MCF7 cell lysate stained with ARG62979 anti-Cytokeratin 19 antibody [A53-B/A2] at 1:500 dilution.
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ARG62979 anti-Cytokeratin 19 antibody [A53-B/A2] IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue stained with ARG62979 anti-Cytokeratin 19 antibody [A53-B/A2].
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ARG62979 anti-Cytokeratin 19 antibody [A53-B/A2] WB image
Western blot: MCF7 cell lysate stained with ARG62979 anti-Cytokeratin 19 antibody [A53-B/A2], in reducing conditions.
客户反馈
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anti-Cytokeratin 19 antibody [A53-B/A2]
Application：WB
Sample：MCF7 cell lysate
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
