ARG66352
anti-Cytokeratin 18 antibody [SQab18102]
anti-Cytokeratin 18 antibody [SQab18102] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab18102] recognizes Cytokeratin 18 |
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| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab18102 |
| 同位型 | IgG |
| 靶点名称 | Cytokeratin 18 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 330-430 of Human Cytokeratin 18. |
| 偶联标记 | Un-conjugated |
| 別名 | Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | HCT116; Human liver tissue | ||||||
| 实际分子量 | ~ 48 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT18 |
| 全名 | keratin 18, type I |
| 背景介绍 | Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt] |
| 产品亮点 | Related products: Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66352 anti-Cytokeratin 18 antibody [SQab18102] WB image (Customer's Feedback)
Western blot: HCT116 cell lysate stained with ARG66352 anti-Cytokeratin 18 antibody [SQab18102] at 1:1000 dilution.
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ARG66352 anti-Cytokeratin 18 antibody [SQab18102] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded liver tissue stained with ARG66352 anti-Cytokeratin 18 antibody [SQab18102]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
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anti-Cytokeratin 18 antibody [SQab18102]
Application：WB
Sample：HCT116
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：Overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
