ARG66971
anti-Cytokeratin 14 + 17 antibody
anti-Cytokeratin 14 + 17 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cytokeratin 14 + 17 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cytokeratin 14 + 17 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the middle region of Human Cytokeratin 14. |
| 偶联标记 | Un-conjugated |
| 別名 | K14; CK-14; Cytokeratin-14; Keratin, type I cytoskeletal 14; EBS3; EBS4; NFJ; Keratin-14; CK14; KRT17; Keratin 17; Keratin, Type I Cytoskeletal 17; Keratin 17, Type I; Cytokeratin-17; CK-17; PCHC1; K17; Keratin-17; PC2; PC |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | MDA-MB-231; Colorectal carcinoma; Breast carcinoma | ||||||||
| 实际分子量 | ~ 45 - 55 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300, 20% Glycerol and 3% BSA. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 20% Glycerol and 3% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KRT14 + KRT17 |
| 全名 | keratin 14 + 17, type I |
| 背景介绍 | Cytokeratin 14 encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008] |
| 生物功能 | The nonhelical tail domain of Cytokeratin 14 is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern. [UniProt] |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG66971 anti-Cytokeratin 14 + 17 antibody ICC/IF image
Immunofluorescence: MDA-MB-231 cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained with ARG66971 anti-Cytokeratin 14 + 17 antibody (red) at 1:150 dilution at 4°C for overnight. DAPI (blue) was used for nuclear staining.
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ARG66971 anti-Cytokeratin 14 + 17 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG66971 anti-Cytokeratin 14 + 17 antibody at 1:100 dilution.
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ARG66971 anti-Cytokeratin 14 + 17 antibody WB image
Western blot: A431 cell lysate stained with ARG66971 anti-Cytokeratin 14 + 17 antibody at 1:2000 dilution, overnight at 4°C.
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ARG66971 anti-Cytokeratin 14 + 17 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colorectal carcinoma tissue stained with ARG66971 anti-Cytokeratin 14 + 17 antibody at 1:100 dilution.
