ARG63933
anti-Cytochrome P450 17A1 antibody
anti-Cytochrome P450 17A1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Cytochrome P450 17A1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cat, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cytochrome P450 17A1 |
| 抗原物种 | Human |
| 抗原 | C-LFKDGDQKLEK |
| 偶联标记 | Un-conjugated |
| 別名 | CYP17; P450C17; Cytochrome P450-C17; S17AH; Cytochrome P450c17; CPT7; Steroid 17-alpha-hydroxylase/17,20 lyase; Steroid 17-alpha-monooxygenase; EC 4.1.2.30; CYPXVII; 17-alpha-hydroxyprogesterone aldolase; EC 1.14.99.9; Cytochrome P450 17A1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P05093 Human Steroid 17-alpha-hydroxylase/17,20 lyase |
|---|---|
| 背景介绍 | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 57 kDa |
| 翻译后修饰 | Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG63933 anti-Cytochrome P450 17A1 antibody WB image
Western blot: Human ovary lysate (35 µg protein in RIPA buffer) stained with ARG63933 anti-Cytochrome P450 17A1 antibody at 0.3 µg/ml dilution.
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ARG63933 anti-Cytochrome P450 17A1 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Adrenal Gland. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63933 anti-Cytochrome P450 17A1 antibody at 3.8 µg/ml dilution followed by AP-staining.
