ARG54065
anti-Cyclin H antibody
anti-Cyclin H antibody for Immunoprecipitation,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes CCNH |
|---|---|
| 反应物种 | Hu |
| 应用 | IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | Cyclin H |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human Cyclin H protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | MO15-associated protein; CAK; CycH; Cyclin-H; p37; p34 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CCNH |
| 全名 | cyclin H |
| 背景介绍 | Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. |
| 生物功能 | Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. [UniProt] |
| 细胞定位 | Nucleus. |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 38 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54065 anti-Cyclin H antibody WB image
Western blot: A431 cell lysate stained with ARG54065 anti-Cyclin H antibody at 1:1000 dilution.
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ARG54065 anti-Cyclin H antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54065 anti-Cyclin H antibody.
