ARG52927
anti-Cyclin E1 antibody
anti-Cyclin E1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cyclin E1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Pig |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cyclin E1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from C-terminus of human cyclin E. |
| 偶联标记 | Un-conjugated |
| 別名 | pCCNE1; CCNE; G1/S-specific cyclin-E1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Breast Carcinoma, Placenta |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunogen affinity purified |
| 缓冲液 | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| 抗菌剂 | < 0.1% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analysis indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. |
| 细胞定位 | Nucleus |
| 产品亮点 | Related Antibody Duos and Panels: ARG30139 Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E) Related products: Cyclin E1 antibodies; Cyclin E1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. |
检测图片 (1) Click the Picture to Zoom In
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ARG52927 anti-Cyclin E1 antibody IHC-P image
Immunohistochemistry: Human breast carcinoma stained with ARG52927 anti-Cyclin E1 antibody.
